An ultra-sensitive ratiometric fluorescent probe for hypochlorous acid detection by the synergistic effect of AIE and TBET and its application of detecting exogenous/endogenous HOCl in living cells

Abstract

An ultra-sensitive and ratiometric fluorescent probe for hypochlorous acid (HOCl) detection based on the mechanism of aggregation induced emission (AIE) and through-bond energy transfer (TBET) has been reported herein. By exploiting the advantages of AIE and TBET, which eliminates emission leakage from dark donors, the probe exhibits ultra-high sensitivity towards HOCl by an enhancement of over 7000-fold in the fluorescence ratio (I589 nm/I477 nm), which is one of the highest recorded so far. The reaction mechanism has been discussed in detail, and the effects of interferents and the reaction kinetics have also been investigated. Lastly, the successful result of exogenous/endogenous HOCl imaging detection in different cell lines indicates the potential use of the probe in living systems.

Ke Cheng, PhD

I am a chemical biologist with expertise in organic chemistry, bioconjugation, protein biology, and oncology. My research focuses on developing small molecule probes and drugs, antibody drug conjugates, and radioligand agents for targeted cancer therapy, including the design of cleavable ADC linkers and next generation payloads, as well as tumor activatable probes and chemoproteomics driven target discovery.